|
課程名稱 |
植物基因轉殖
PLANT GENE TRANSFER |
|
開課學期 |
97-1 |
|
授課對象 |
生物資源暨農學院 園藝暨景觀學系 |
|
授課教師 |
黃鵬林 |
|
課號 |
HORT5024 |
|
課程識別碼 |
628 U1560 |
|
班次 |
|
|
學分 |
3 |
|
全/半年 |
半年 |
|
必/選修 |
選修 |
|
上課時間 |
星期三2,3,4(9:10~12:10) |
|
上課地點 |
園藝R106 |
|
備註 |
第二群必選，三選一。
總人數上限：30人 |
|
Ceiba 課程網頁 |
http://ceiba.ntu.edu.tw/971pgt |
|
課程簡介影片 |
|
|
核心能力關聯 |
本課程尚未建立核心能力關連 |
|
課程大綱
|
|
為確保您我的權利,請尊重智慧財產權及不得非法影印
|
|
課程概述 |
1. Introduction 2.
Agrobacterium tumefaciens 3.
Agrobacterium-mediated
transformation 4. DNA-
mediated gene transfer 5.
Alternative DNA transfer
methods 6. Virus vectors and
their potential application
in plant genetic engineering
7. Genetic manipulation of
the chloroplast genome 8.
Reporter genes 9. Antisense
RNA technology and ribozyme
10. Manipulation of floral
pigmentation genes 11.
Herbicide resistance 12.
Strategies for disease
resistance in plants 13.
Molecular strategy to modify
genes involved in ethylene
biosynthesis. |
|
課程目標 |
本課程涵蓋植物組織培養(plant tissue culture)及重組DNA技術 (recombinant DNA technology)，探討植物基因轉殖之理論、方法與實際，並闡述轉殖植物 (transgenic plants) 之應用途徑。 |
|
課程要求 |
先修科目：生物化學、基礎分子生物學 |
|
預期每週課後學習時數 |
|
|
Office Hours |
另約時間 |
|
指定閱讀 |
|
|
參考書目 |
General
1.Bennett AB & O'Nell SD
(eds) (1990) Horticultural
Biotechnology. Wiley-Liss,
Inc., New York. 387pp.
2.Chawla HS (2002)
Introduction to Plant
Biotechnology. 2nd ed.
Science Publishers, Inc.,
Enfield, NH. 538pp.
3.Clark DP (ed) (2005)
Molecular Biology. Elsevier
Academic Press. 784pp
4.Collins GB & Shepherd RJ
(eds) (1996) Engineering
Plants for Commercial
Products and Applications.
Annals of the New York
Academy of Sciences, Volume
792. 183pp.
5.Dennis ES, Hohn B, Hohn T,
King PJ, Schell J & Verma
DPS (eds) (1991) Plant Gene
Research. Springer-Verlag
Wien, New York. 166pp.
6.Fowler MW & Warren GS
(eds) (1992) Plant
Biotechnology. Pergamon
Press. 366pp.
7.Gibson G & Muse SV (2002)
A Primer of Genome Science.
Sinauer Associates Inc.
Sunderland, Massachusetts.
347pp.
8.Glick BR & Pasternak JJ
(2003) Molecular
Biotechnology. American
Society for Microbiology,
Washington, DC. 760 pp.
9.Grierson D & Covey SN
(1988) Plant Molecular
Biology. 2nd ed. Chapman and
Hall, New York. 233pp.
10.Grotewold E (2003) Plant
Functional Genomics. Humana
Press, Totowa, New Jersey.
449pp.
11.Hiatt A (ed) (1993)
Transgenic Plants. Marcel
Dekker, Inc. 340pp.
12.Kung SD & Arntzen CJ
(eds) (1989) Plant
Biotechnology. Butterworths
Publishers. Boston. 423pp.
13.Lal R & Lal S (1990) Crop
Improvement utilizing
Biotechnology. CRC Press,
Inc. Boca Raton, Florida.
353pp.
14.Lamb CJ & Beachy RN (eds)
(1990) Plant Gene Transfer.
Alan R. Liss, Inc. 345pp.
15.Lewin, B. 2008. Genes IX.
Jones and Bartlett
Publishers, Inc. Sudbury,
Massachusetts, USA. 892 pp.
16.Lycett GW & Grieson D
(1990) Genetic Engineering
of Crop Plants.
Butterworths. 293pp.
17.Owen MRL & Pen J (1996)
Transgenic Plants: A
Production Sysetm for
Industrial and
Pharmaceutical Proteins.
John Wiley & Sons Ltd, New
York. 348pp.
18.Ramawat KG (ed) (2004)
Biotechnology of Medicinal
Plants – Vitalizer and
Therapeutic. Science
Publishers, Inc. 302pp.
19.Slater A, Scott NW &
Fowler MR (2003) Plant
Biotechnology – The Genetic
Manipulation of Plants.
Oxford University Press.
346pp.
20.You CB, Chen ZL & Ding Y
(1992) Biotechnology in
Agriculture. Proceedings of
the First Asia-Pacific
Conference on Agricultural
Biotechnology (Beijing,
China, 1992). Kluwer
Academic Publishers,
Dordrecht, Boston, London.
519pp.
Lab manual
1.Channarayappa (2007)
Molecular Biotechnology –
Principles and Practices.
Universities Press.
Hyderabad, India. 1217pp.
2.Draper J, Scott R,
Armitage P & Walden R (eds)
(1988) Plant Genetic
Transformation and Gene
Expression - A laboratory
manual. Blackwell Scientific
Publications. Oxford. 355pp.
3.Gartland KMA & Davey MR
(eds) (1995) Agrobacterium
Protocols. Humana Press.
Totowa, New Jersey. 417pp.
4.Lindsey K (ed) (1993)
Plant Tissue Culture Manual -
Supplement 3. Kluwer
Academic Publishers,
Dordrecht, Boston, London.
5.Martinez-Zapater JM &
Salinas J (1998) Arabidopsis
Protocols. Humana Press.
Totowa, New Jersey. 440pp.
6.Shaw CH (ed) (1988) Plant
Molecular Biology -A
practical approach. IRL
Press. Oxford. Washington
DC. 313pp.
7.Tagu D & Moussard C (ed)
(2006) Techniques for
Molecular Biology. Science
Publishers, Enfield, NH,USA.
227pp.
8.Weigel D & Glazebrook J
(2002) Arabidopsis: A
laboratory manual. Cold
Spring Harbor Laboratory
Press. New York. 354pp. |
|
評量方式
(僅供參考) |
|
No. |
項目 |
百分比 |
說明 |
|
1. |
期中考 |
33% |
|
2. |
文獻導讀或期末考 |
33% |
|
3. |
隨堂測驗 |
0% |
|
4. |
作業 |
0% |
|
5. |
報告 |
34% |
|
|
|
週次 |
日期 |
單元主題 |
|
第1週 |
9/17 |
Introduction |
|
第2週 |
9/24 |
Possible risks and safety assessments of transgenic plants |
|
第3週 |
10/01 |
Agrobacterium tumefaciens |
|
第4週 |
10/08 |
Agrobacterium-mediated transformation |
|
第5週 |
10/15 |
DNA-mediated gene transfer |
|
第6週 |
10/22 |
Alternative DNA transfer methods |
|
第7週 |
10/29 |
Virus vectors and their potential application in plant genetic engineering |
|
第8週 |
11/05 |
Genetic manipulation of the chloroplast genome |
|
第9週 |
11/12 |
期中考 |
|
第10週 |
11/19 |
Reporter genes |
|
第11週 |
11/26 |
Antisense RNA technology and ribozyme |
|
第12週 |
12/03 |
Manipulation of floral pigmentation genes |
|
第13週 |
12/10 |
Herbicide resistance |
|
第14週 |
12/17 |
Strategies for disease resistance in plants |
|
第15週 |
12/24 |
Gene silencing and RNA interference |
|
第16週 |
12/31 |
Molecular strategy to modify genes involved in ethylene biosynthesis |
|
第17週 |
1/07 |
文獻導讀 |
|